Item # 1101-F: Fluorescene Murine Anti-gp120 HIV-1 IIIB mAb IgG
Mass/vial: Approximately 50µg IgG
Diluent: 10mM Tris, pH 8.0
F/P Ratio: 3 - 4
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 50
Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence
Description: Fluorescene conjugated Murine Anti-gp120, V3 loop specific (HIV-1 IIIB) mAb.
Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This fluorescene conjugated mAb binds to native and recombinant HIV-1 gp120 in Dot Blot assays and stains cell surfaces in direct immunofluorescence assays.
Biological Activity: None
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified gp120 may be performed in 1 : 200 dilution range.