Fluorescene Murine mAb gp120 Cross Reactive

ImmunoDXSKU: 1121-F


Product Specifications:

Item # 1121-F: Fluorescene Murine Anti-gp120 HIV-1 mAb IgG (Cross Reactive)


Mass/vial: Approximately 50µg IgG2b


Vol/vial: 100ul


Diluent: 10mM Tris, pH 8.0                                  


Stabilizer: None                                                       


Preservative: None                                                       


Storage: -75°C                                                                             


F/P Ratio: 3 - 4


Physical State: Frozen Liquid


Stability: At least 12 months at -75°C


Minimum Dilution: 1 : 50


Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.


Description: Fluorescene conjugated Murine Anti-gp120, V3 loop specific, HIV-1 cross reactive mAb.


Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.


Specificity: This fluorescene conjugated mAb binds to native and recombinant HIV-1 gp120 (IIIB, MN, SF2, RF, CDC4) in Dot Blot assays and stains cell surface bound gp120 in HIV-1 infected SupT1 and primary HIV-1 infected human PBLs.


Biological Activity: None


Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range.  Dot Blot assays with purified gp120 may be performed in 1 : 200 dilution range.

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