Item # 1121-F: Fluorescene Murine Anti-gp120 HIV-1 mAb IgG (Cross Reactive)
Mass/vial: Approximately 50µg IgG2b
Diluent: 10mM Tris, pH 8.0
F/P Ratio: 3 - 4
Physical State: Frozen Liquid
Stability: At least 12 months at -75°C
Minimum Dilution: 1 : 50
Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.
Description: Fluorescene conjugated Murine Anti-gp120, V3 loop specific, HIV-1 cross reactive mAb.
Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This fluorescene conjugated mAb binds to native and recombinant HIV-1 gp120 (IIIB, MN, SF2, RF, CDC4) in Dot Blot assays and stains cell surface bound gp120 in HIV-1 infected SupT1 and primary HIV-1 infected human PBLs.
Biological Activity: None
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified gp120 may be performed in 1 : 200 dilution range.