Product Specifications:
Item# 5001: Recombinant SIVmac251 Glycoprotein gp130 (Baculovirus)
Concentration: See vial
Mass/vial: 100µg
Volume/vial: see vial
Diluent: PBS
Purity: >95%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: At least 12 months at -75°C.
Application: Immunization, CD4 Binding, B & T-Cell Activation
Description: Full length Recombinant SIV gp130 glycoprotein produced in the Baculovirus Expression System.
Purification: This protein is purified by immunoaffinity chromatography to >90% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and simian serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp130/Anti-gp130 mAb-peroxidase capture ELISA. This protein activates simian T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.
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