ITEM# 1081: Recombinant HIV-1 ADA Envelope Glycoprotein gp120 ( Baculovirus)
Concentration: See vial
Mass/vial See vial Diluent PBS
Physical State: Frozen Liquid
Stability: At least 24 months at -75oC.
DESCRIPTION: Gene cloned from M cell-tropic HIV-1 ADA strain and the full length recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression System.
PURIFICATION: This protein is purified by immuno-affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
BIOLOGICAL ACTIVITY: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti gp120 mAb-peroxidase capture ELISA. This protein activates human monocytic cells (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
CD4 Binding, Cell activation, In-Vitro Diagnostics.
INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications
ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.