Item# 1006-B: Biotin rp66 (RT) HIV-1 IIIB
Vol./Vial: 50µl +
Stabilizer: 1% BSA
Stability: 6 Months
Minimum Dilution: 1 : 2000
Application: Western ELISA, ELISA, T-Cell Research, Drug Screening.
Description: Biotin conjugated to rp66 (RT).
Coupling Ratio: Approximately 5-10 moles of biotin per mole rp66 (RT)
Specificity: Biotin rp66 (RT) binds strepavidin-HRP in ELISA and Western ELISA.
Biological Activity: Biotin rp66 (RT) binds to solid phase anti-p66 murine monoclonal antibodies and human anti-p66 polyclonal antibodies as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. rp66 (RT) capture ELISA may be performed in 100- 500ng antibody in solid phase and 1-1000 dilution of biotin-rp66 (RT).