Item# 1004-B: Biotin rgp160 HIV-1 IIIB
Vol./Vial: 50µl +
Stabilizer: 1% BSA
Stability: 6 Months
Minimum Dilution: 1 : 5 000
Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA.
Description: Biotin conjugated to gp160.
Coupling Ratio: Approximately 10-20 moles of biotin per mole gp160.
Specificity: Biotin gp160 binds strepavidin-AP/HRP in ELISA and Western ELISA.
Biological Activity: Biotin gp160 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. gp160/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and 1-10ng of biotin-gp160.