Item#1007: Recombinant HIV-1 vif (E.coli)
Concentration: See vial
Diluent: 50mM Tris, pH8, 150mM NaCl
Physical State: Frozen Liquid
Stability: At least 24 months at -75°C.
Application: ELISA, Western ELISA, Immunological Studies, Drug Screening.
Description: Full length Recombinant HIV-1 HXB2 vif produced as a 6xHis-fusion protein in E.coli.
Purification: This recombinant protein is purified by Nickle -Affinity chromatography and the purified protein is cleaved to produce vif of >99% purity, as determined by SDSPAGE, reduced.
Molecular Weight: 23kD.
Specificity: This protein shows reactivity with murine monoclonal antibodies and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity:Vif dependent HIV-1 virus production in HIV-1 infected SupT1, Jurkat and CEM cells
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.