vif HIV-1 (E. coli)

SKU: 1007

Product Specifications:

Item#1007: Recombinant HIV-1 vif (E.coli)


Concentration: See vial


Mass/vial: 100ug


Volume/vial: 1ml


Diluent: 50mM Tris, pH8, 150mM NaCl


Purity: >95%


Stabilizer: None


Preservative: None


Storage: -75°C


Physical State: Frozen Liquid


Stability: At least 24 months at -75°C.


Application: ELISA, Western ELISA, Immunological Studies, Drug Screening.


Description: Full length Recombinant HIV-1 HXB2 vif produced as a 6xHis-fusion protein in E.coli.


Purification: This recombinant protein is purified by Nickle -Affinity chromatography and the purified protein is cleaved to produce vif of >99% purity, as determined by SDSPAGE, reduced.


Molecular Weight: 23kD.


Specificity: This protein shows reactivity with murine monoclonal antibodies and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.


Biological Activity:Vif dependent HIV-1 virus production in HIV-1 infected SupT1, Jurkat and CEM cells


Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.