vif HIV-1 (E. coli)

$545.00
SKU: 1007

Product Specifications:

Item#1007: Recombinant HIV-1 vif (E.coli)

 

Concentration: See vial

 

Mass/vial: 100ug

 

Volume/vial: 1ml

 

Diluent: 50mM Tris, pH8, 150mM NaCl

 

Purity: >95%

 

Stabilizer: None

 

Preservative: None

 

Storage: -75°C

 

Physical State: Frozen Liquid

 

Stability: At least 24 months at -75°C.

 

Application: ELISA, Western ELISA, Immunological Studies, Drug Screening.

 

Description: Full length Recombinant HIV-1 HXB2 vif produced as a 6xHis-fusion protein in E.coli.

 

Purification: This recombinant protein is purified by Nickle -Affinity chromatography and the purified protein is cleaved to produce vif of >99% purity, as determined by SDSPAGE, reduced.

 

Molecular Weight: 23kD.

 

Specificity: This protein shows reactivity with murine monoclonal antibodies and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.

 

Biological Activity:Vif dependent HIV-1 virus production in HIV-1 infected SupT1, Jurkat and CEM cells

 

Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.