Toxoplasma Composite 1 Antigen GRA1-GRA3-GRA6-GRA7-M2AP (Baculo)

ImmunoDXSKU: 60011


Product Specifications:

Item# 60011: Recombinant Toxoplasma GRA1,3,6,7, M2AP Antigen (Baculo)


Concentration: See vial                                                             


Mass/vial: 1mg                                                  


Volume/vial: 500ul                                                          


Diluent: 50mM Tris,20mM Nacl, SDS trace, pH8+-                                                            


Purity: >95%                                                                                                    


Stabilizer: None                                                                        


Preservative: None                                                                     


Storage:   long term -75°C, Short term-20C°


Physical State:Frozen Liquid


Stability:  2 years at -75°C 


Application: In-Vitro Diagnostics, Immunization


Description: Recombinant Toxoplasma GRA1,3,6,7, M2AP Antigen produced in the Baculovirus Expression  System.  


molecular Weight: 62kD.


Purification: This protein is purified by solvent extraction and preparatice electrophoresisi to >95% purity as determined by SDS-PAGE, reduced.


Specificity: This protein binds to Toxoplasma converted human serum polyclonal antibodies (IgG,IgM) in ELISA and  Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests.


Biological Activity:  Not done


Applications and Instructions for use


Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.

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