Item# 15005: Recombinant Treponema pallidum 17kD Membrane Protein (E.coli)
Diluent: 10 mM Tris, pH 8.0, SDS (<0.01%)
Physical State:Frozen Liquid
Stability: 2 years at -75°C
Application: In-Vitro Diagnostics, Immunization
Description: Recombinant Treponema pallidum 17Kd protein produced as a gst-fusion protein in the E.coli Expression System. Fusion protein protein
molecular Weight: 42kD.
Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine anti-TP17 kD protein monoclonal antibodies and T. pallidum converted human serum polyclonal antibodies in ELISA and Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests.
Biological Activity: Not done
Applications and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.