Item# 63001: Recombinant West Nile Envelope E Protein (E.coli)
Concentration: See vial
Mass/vial: See vial
Diluent: 50mM Tris, 0.02% SDS, pH8
Physical State: Frozen Liquid
Applications: ELISA, Western Blot, West Nile Diagnostics
Description: Synthetic West Nile full length envelope E protein DNA sequence, expressed as a his-tag protein in the E.coli Expression System.
Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 56.1kD
Specificity: This protein binds to murine monoclonal antibodies- structural epitopes, and to West Nile virus converted horse sera as determined by Elisa and Western blot assays.
Biological Activity: Not Determined.
Application and Instructions for use:
Recommended concentrations for use are approximate values. Elisa is performed in 1-2uglml protein range for titeringvirus converted horse serum polyclonal antibodies.