rtat HIV-1 MN (E. coli)

SKU: 1032

Product Specifications:

Item# 1032:  Recombinant tat HIV-1 MN


Concentration: See vial                                                        


Mass/vial: 100ug                                                       


Volume/vial: See vial                                                           


Diluent: 0.2 M KCl, 5mM Glutathione                    


Purity: >95%                                                                                  


Stabilizer: None                                                                        


Preservative: None                                                                     


Storage: -75°C


Physical State: Frozen Liquid


Stability: At least 6 months at -75°C.             


Application: ELISA, Western ELISA, Anti-tat Drug Screening, Immunization, Transcriptional Activation.                                             

Description: Full length (101 amino acids) Recombinant HIV-1 MN tat produced in the E.coli Expression System.


Purification: This protein is purified by ion affinity and reverse phase HPLC to >95% purity, as determined by SDS-PAGE and HPLC.


Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies (HIV-1 converted serum)  in ELISA and Western ELISA.


Biological Activity:  The biological specificity of this protein was determined by LTR-Beta galactosidase induction in MAGI and MAGI-CCR5 cells. Tat in 1-10ug/ml range induced Beta-galactosidase activity 30-fold over un-treated cells.


Endotoxin: Less than 0.01 EU/mg of protein as determined by BioWhittaker Kinetic QCL Kit.


Application and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.  Dilute tat stock solution in saline-phosphate buffer (150mM NaCl, 50mM sodiumphosphate, pH 6.5) immediately before use.  Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity. Transcriptional activation assays with tat are performed in 1-10µg/ml range.  ELISA and Western ELISA require tat in 10-100ng protein range.