rtat HIV-1 Bal (E. coli)

SKU: 1052

Product Specifications:

Item#1052:  Recombinant tat HIV-1 Bal (E.coli)

 Concentration: 1mg/ml                                                      

 Mass/vial: 100ug                                          

 Volume/vial: See vial                                                           

 Diluent: 50mM Tris, 0.1M NaCl, 2mM dtt, pH7.8                    

 Purity: >95%                                                                                  

 Stabilizer: None                                                                        

 Preservative: None                                                                     

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: At least 6 months at -75°C.             

 Application: ELISA, Western ELISA, Anti-tat Drug Screening, Immunization, Transcriptional Activation.                                             

Description: Full length (101 amino acids) Recombinant HIV-1 Bal tat produced in the E.coli Expression System.

 Purification:  This protein was  purified by solvent extraction, ion affinity and macro-molecular sieving  to approx.95% purity, as determined by SDS-PAGE, reduced.

 MW: approx.16kD.

 Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies in ELISA and Western ELISA.

 Biological Activity: Not Done

 Endotoxin: Assay pending

 Application and Instructions for use

 Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.  Dilute tat stock solution in saline-phosphate buffer (150mM NaCl, 50mM sodiumphosphate, pH 6.5) immediately before use.  Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity. Transcriptional activation assays with tat are performed in 1-10µg/ml range.  ELISA and Western ELISA require tat in 10-100ng protein range.