Item#1052: Recombinant tat HIV-1 Bal (E.coli)
Volume/vial: See vial
Diluent: 50mM Tris, 0.1M NaCl, 2mM dtt, pH7.8
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C.
Application: ELISA, Western ELISA, Anti-tat Drug Screening, Immunization, Transcriptional Activation.
Description: Full length (101 amino acids) Recombinant HIV-1 Bal tat produced in the E.coli Expression System.
Purification: This protein was purified by solvent extraction, ion affinity and macro-molecular sieving to approx.95% purity, as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Not Done
Endotoxin: Assay pending
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute tat stock solution in saline-phosphate buffer (150mM NaCl, 50mM sodiumphosphate, pH 6.5) immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity. Transcriptional activation assays with tat are performed in 1-10µg/ml range. ELISA and Western ELISA require tat in 10-100ng protein range.