Item#40001: Recombinant HBV Surface Antigen Ay (E.coli)
Concentration: See vial
Diluent: 10mMNa-PO4, 50mM NaCl, pH 6.8 10% Glycerol
Purity: Approx. 92%
Physical State: Aqueous Solution
Stability: 24 Months at -75oC.
Applications: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 0.1-1.0µg protein depending on the nature and affinity of the secondary detection reagent. Studies with HBV convereted human sera are performed in 1:200 to 1:10,000 antibody dilution range.
Description: Recombinant HBV surface protein Ay expressed in the E.coli Expression system.
Purification: Proprietary. Purity approx. 92% as determined by SDS_PAGE, reduced. MW: 22kD
Specificity: Strongly binds to HBV converted human serum antibodies as determined by ELISA and Western ELISA. Protein is suitable for lateral flow diagnostic assays.
CHO- expressed HIV-1IIIB rgp120 (2.5 μg) and biotinylated cyclic V2-TH023 peptide (1 μg and 5 μg) were incubated with RPMI8866 cells