Item # 4001-10: Recombinant HCV 70kD E2 Envelope Protein (Baculovirus) AP
Concentration: See vial
Purity : Approx. 95%
Physical State: Frozen Liquid
Stability: At least 2 years at -75°C.
Applications: In-Vitro HCV Diagnostics, T-Cell Activation, Immunization.
Description: Full length Recombinant HCV 70kD envelope- GST fusion protein produced in the Baculovirus Expression System.
Purification: This protein is purified by glutathione affinity chromatography to >90% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HCV converted human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Not determined.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.