Item# 19002: Recombinant Rubella E2 Envelope Protein
Concentration: See vial
Volume/vial: See vial
Diluent: 50mM NaPO4, 0.15M NaCl, pH8
Physical State: Frozen Liquid
Stability: Atleast 24 months at -75°C
Applications: In-Vitro Diagnostics, Immunization, T-Cell Activation. (Test pending)
Description: Recombinant Rubella E2 envelope protein produced as a fusion protein in the Eukaryotic Expression System.
Purificatiuon: This protein is purified by affinity chromatography and by preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 33kD
Specificity: This protein binds to goat anti- Rubella polyclonal antibodies and Rubella converted human serum polyclonal antibodies in ELISA (IgG, IgM) and Western Elisa. Antigen tested by dot blots and lateral flow immunochromatographic tests for diagnostic use.
Biological Activity: Not done
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.