Item# 1008-B: Biotin rnef HIV-1 IIIB
Stabilizer: 1% BSA
Stability: 6 Months
Minimum Dilution: 1 : 2000
Application: T-Cell Research, ELISA, Western ELISA, Drug Screening, Diagnostics.
Description: Biotin conjugated to nef HIV-1 (E.coli).
Coupling Ratio: Approximately 5-10 moles of biotin per mole nef.
Biological Activity: Biotin nef binds to solid phase murine anti-nef mAb as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. nef capture ELISA may be performed in 100ng anti-nef mAb in solid phase and 1-10ng of biotin-nef.