Item# 1006-10: Recombinant HIV-1 IIIB p66 (RT)
Concentration: See vial
Volume/vial: See vial
Diluent: 50mM Tris,pH8,10% Glycerol 1mM DTT,0.2M NaCl
Physical State: Frozen Liquid
Stability: At least 24 months at -75°C.
Application: ELISA, Western ELISA, Reverse Trascriptase Reference, Drug Screening.
Description: Full length Recombinant heterodimeric HIV-1 IIIB p66 (RT) produced in E.coli expression system.
Purification: This heterodimeric protein is purified by ion-affinity chromatography to >98% purity as determined by SDS-PAGE, reduced: apparent MW= 66kD.
Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: 0.1 unit of purified RT incorporates a minimum of 5 picomoles of dTTP per minute under standard RT assay conditions.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody. Solid phase antigen in 50-100ng amounts shows reactivity with human serum antibodies in dot blot assays.