rp66 (RT) HIV-1 IIIB (E. coli)

SKU: 1006

Product Specifications:

Item# 1006-10: Recombinant HIV-1 IIIB p66 (RT)


Concentration: See vial


Mass/vial: 1mg


Volume/vial: See vial


Diluent: 50mM Tris,pH8,10% Glycerol 1mM DTT,0.2M NaCl


Purity: >95%


Stabilizer: None


Preservative: None


Storage: -75°C


Physical State: Frozen Liquid


Stability: At least 24 months at -75°C.


Application: ELISA, Western ELISA, Reverse Trascriptase Reference, Drug Screening.


Description: Full length Recombinant heterodimeric HIV-1 IIIB p66 (RT) produced in E.coli expression system.


Purification: This heterodimeric protein is purified by ion-affinity chromatography to >98% purity as determined by SDS-PAGE, reduced: apparent MW= 66kD.


Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA.

Biological Activity: 0.1 unit of purified RT incorporates a minimum of 5 picomoles of dTTP per minute under standard RT assay conditions.

Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody. Solid phase antigen in 50-100ng amounts shows reactivity with human serum antibodies in dot blot assays.