Item# 1005-10: Recombinant p17 HIV-1 Core Protein
Concentration: See vial
Diluent: MES Buffer, pH. 6.5
Physical State: Frozen Liquid
Stability: At least 24 months at -75°C.
Application: ELISA, Western ELISA, Diagnostics
Description: Recombinant HIV-1 p17 is expressed as a Glutathione S-transferase (GST) fusion protien produced in the E.coli expression system.
Purification: This protein is purified by GST-agarose affinity chromotography and p17 is cleaved from the fusion protein by using Factor Xa. GST is removed from the mixture by a second passage through GST-agarose.
Specificity: This protein binds to HIV-1 positive human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Recombinant p17 core protein contains authentic N and C termini which is normally processed from the sequence of the matrix antigen with relevant HIV-1 protease.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute p17 stock solution in MES buffer immediately before use. ELISA assays may be performed with p17 in solid phase in 10-100ng range. Western ELISA may be performed in 100-500ng depending on the specificity of the primary antibody used in the test system.