rnef SIV mac251 (E.coli)

SKU: 5008
Size: 100 ug

Product Specifications:

Item# 5008: Recombinant SIVmac251 nef (Baculovirus) 


Concentration: See vial


Mass/vial: 100µg  


Diluent: 50mM Tris, pH8, 150mM NaCl  


Purity : >95% 


Stabilizer: None


Preservative: None


Storage: -75°C


Physical State: Frozen Liquid


Stability: At least 6 months at -75°C.


Applications: Cell Activation,Immunological Studies, Drug Screening, ELISA / Western ELISA, Diagnostics


Description: Full length Recombinant SIV mac251 nef produced as a GST fusion protein in the Baculovirus Expression System.


Purification: This recombinant protein is purified by Glutathione -affinity chromatography and the purified protein is thrombin-cleaved to produce nef of >95% purity, as determined by SDS-PAGE, reduced.


Specificity: This protein shows reactivity with murine monoclonal antibodies and SIV converted serum polyclonal antibodies in ELISA and Western ELISA.


Biological activity: Not done.


Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.