Recombinant HIV-1 nef (E.coli)

SKU: 1008

Product Specifications:

Item# 1008: Recombinant HIV-1 nef (E.coli)

 Concentration: See vial 

 Mass/vial: 100 ug  

 Volume/vial: 100ul  

 Diluent: 50mM Tris, pH8, 150mM NaCl

 Purity: >95%

 Stabilizer: None

 Preservative: None

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: Minimum 12 months at -75°C.

 Application: Diagnostics, ELISA/ Western ELISA, Drug Screening, Immunological Studies.

 Description: Full length Recombinant HIV-1 (LAV) nef produced as a GST fusion protein in E.coli.

 Purification: This recombinant protein is purified by Glutathion -affinity chromatography and the purified protein is thrombin-cleaved to produce nef of >95% purity, as determined by SDS-PAGE, reduced.

 Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA.

 Biological Activity: Not done.

 Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.