rnef HIV-1 (E.coli)

SKU: 1008

Product Specifications:

Item# 1008: Recombinant HIV-1 nef (E.coli)


Concentration: See vial 


Mass/vial: 100 ug  


Volume/vial: 100ul  


Diluent: 50mM Tris, pH8, 150mM NaCl


Purity: >95%


Stabilizer: None


Preservative: None


Storage: -75°C


Physical State: Frozen Liquid


Stability: Minimum 12 months at -75°C.


Application: Diagnostics, ELISA/ Western ELISA, Drug Screening, Immunological Studies.


Description: Full length Recombinant HIV-1 (LAV) nef produced as a GST fusion protein in E.coli.


Purification: This recombinant protein is purified by Glutathion -affinity chromatography and the purified protein is thrombin-cleaved to produce nef of >95% purity, as determined by SDS-PAGE, reduced.


Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA.


Biological Activity: Not done.


Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.