Item# 1008: Recombinant HIV-1 nef (E.coli)
Concentration: See vial
Mass/vial: 100 ug
Diluent: 50mM Tris, pH8, 150mM NaCl
Physical State: Frozen Liquid
Stability: Minimum 12 months at -75°C.
Application: Diagnostics, ELISA/ Western ELISA, Drug Screening, Immunological Studies.
Description: Full length Recombinant HIV-1 (LAV) nef produced as a GST fusion protein in E.coli.
Purification: This recombinant protein is purified by Glutathion -affinity chromatography and the purified protein is thrombin-cleaved to produce nef of >95% purity, as determined by SDS-PAGE, reduced.
Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Not done.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.