PRODUCT# 105-40: Recombinant M. tuberculosis MTP-64 Antigen (E.coli)
Concentration: see vial ELISA
Mass/vial: see vial Western ELISA
Diluent: 50mMTris, 0.1M Nacl, pH 8.5 TB Diagnostics
Purity: >98% Immunization
Physical State: Frozen Liquid
Stability: 24 months at 75oC.
DESCRIPTION: M. tuberculosis (virulent strain) MTP-64 antigen – synthetic gene -produced in the E. coli expression system.
PURIFICATION: Purified by His Select affinity chromatography to >98% purity as determined by SDS-PAGE, reduced. MW: approx. 33.9kD
SPECIFICITY: This MTP-64 protein reacts with M. tuberculosis converted human serum in ELISA and Western ELISA. Suitable for diagnostic applications.
BIOLOGICAL ACTIVITY: Not done
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.