rMalaria HRP II Antigen (E.coli)

ImmunoDXSKU: 20050


Product Specifications:

Item# 20050: Recombinant Malaria  pf HRP II Antigen (E.coli)                    


Concentration:See vial                                                         


Mass/vial:  See vial                                                                                        


Diluent: 50mM Tris, pH8, 2mM DTT  0.02%SDS                                                                         


Purity: >95%                                                                       


Stabilizer: None                                                                        


Preservative: None


Storage: -75°C


Physical State: Frozen Liquid


Stability: At least 36 months. 


Application: ELISA, Western ELISA, Diagnostics.


Description: Synthetic malaria parasite pf HRP II DNA sequence, expressed as C-terminus 4 his protein in the E.coli Expression System


Purification:This protein was purified by aqueous solvent extraction Ni-affinity and preparative electrophoresis to  >98% purity as determined by SDS-PAGE, reduced.


Molecular Weight: approx. 40kD.


Specificity: This protein binds to Anti HRP II  murine monoclonal antibodies IgG and IgM and to malaria parasite pf HRP II converted human sera as determined by Elisa and  Western Blots assays. The antigen is suitable for one-step lateral flow diagnostic testing.

Application and Instructions for use

Recommended concentrations for use are approximate values.  Elisa is performed in 1-2ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Concentarions if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.

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