Item# 20050: Recombinant Malaria pf HRP II Antigen (E.coli)
Mass/vial: See vial
Diluent: 50mM Tris, pH8, 2mM DTT 0.02%SDS
Physical State: Frozen Liquid
Stability: At least 36 months.
Application: ELISA, Western ELISA, Diagnostics.
Description: Synthetic malaria parasite pf HRP II DNA sequence, expressed as C-terminus 4 his protein in the E.coli Expression System
Purification:This protein was purified by aqueous solvent extraction Ni-affinity and preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 40kD.
Specificity: This protein binds to Anti HRP II murine monoclonal antibodies IgG and IgM and to malaria parasite pf HRP II converted human sera as determined by Elisa and Western Blots assays. The antigen is suitable for one-step lateral flow diagnostic testing.
Application and Instructions for use
Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Concentarions if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.