Item# 20060: Recombinant Malaria pLDH pv Antigen (E.coli)
Mass/vial: See vial
Diluent: 50mM NaCl, 50mM Tris, pH8.9
Physical State: Frozen Liquid
Stability: At least 36 months.
Application: ELISA, Western ELISA, Diagnostics.
Description: Synthetic Malaria parasite LDH pv DNA sequence, expressed as a his-tag protein in the E.coli Expression System. Full length 346 amino acids protein.
Purification: This protein was purified by aqueous solvent extraction NiNTA affinity chromatography to >95% purity as determined by SDS-PAGE, reduced
Molecular Weight: approx. 77kD.
Specificity: This protein binds to murine monoclonal antibodies- structural epitopes, rabbit polyclonal antibodies and to malaria parasite converted human sera as determined by Elisa and Western Blots assays.
Application and Instructions for use
Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Concentarions if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.
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