rhT-Cell Co-Receptor CD4

$3,055.00
SKU: 7001-10

Product Specifications:

Item# 7001-2: Recombinant Human T-Cell Receptor sCD4 

 

Concentration: See vial 

 

Mass/vial: 1mg 

 

Volume/vial: 1ml 

 

Diluent: PBS 

 

Purity: >98%

 

Stabilizer: None

 

Preservative: None

 

Storage: -75°C

 

Physical State: Frozen Liquid

 

Stability: At least 24 months at -75°C

 

Applications: T-Cell Research,Human Diagnostics, HIV-1 Neutralization, Drug Screening

 

Description: Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4.

 

Purification:This protein is purified by immunoaffinity and gel exclusion chromatography to >98% purity as determined by SDS PAGE, reduced.

 

Molecular weight: Approximately 55kD.

 

Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA.

 

Biological Activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs.

 

Application and Instructions for use:

 

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s).

2. Recommended concentration for virus neutralization assays are in 1-10µg/ml of the HIV-1 infected culture.

3. Drug screening assays are performed with 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using murine anti-gp120-peroxidase conjugate (catalog# 1121-P) or directly using HIV-1 rgp120-peroxidase conjugate (catalog# 1001-P).