rHBV Core Antigen (E.coli)

$750.00
SKU: 40003
Size: 1 mg

PRODUCT SPECIFICATIONS

 

PRODUCT# 40003:  Recombinant HBV Core Antigen

 

 

SPECIFICATIONS                                                               APPLICATIONS

Concentration:

1mg/ml

Diagnostics

Mass/vial:

1mg

Immunization

Volume/vial:

100ul

T-Cell Activation

Diluent:

10mMNa-PO4, 20mM NaCl, pH 7

 

Purity:

>95%

 

Stabilizer:

None

 

Preservative:

None

 

Storage:

-75C

 

Physical State:

Aqueous Solution

 

Stability:

24  Months at -75oC

 

 

 

 

 

DESCRIPTION:

Recombinant HBV core protein -183 amino acids- expressed in the E.coli Expression system.

 

 

PURIFICATION:

Proprietary. Purity > 95 % as determined by SDS_PAGE, reduced. MW: approx. 25kD

 

SPECIFICITY:

Strongly binds to HBV converted human serum antibodies as determined by  ELISA and Western ELISA. Protein is suitable for lateral flow diagnostic assays.

 

 

BIOLOGICAL ACTIVITY:

Not determined.

 

 

APPLICATION AND INSTRUCTIONS FOR USE

 

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

 

ELISA and Western ELISA require 0.1-1.0µg protein depending on the nature and affinity of the secondary  detection reagent.  Studies with HBV convereted human sera are performed in 1:200 to 1:10,000 antibody dilution range.