PRODUCT# 7015 : Recombinant Human APOBEC3G Protein (Baculo)
Concentration: See vial Vif binding assays
Mass/vial: 0.1mg RNA Binding
Diluent : 50mM Sodium Phospahte, pH 8.0
0.1M NaCl, Brij-35 0.01%
Purity : >95%
Physical State: Liquid
Stability: Not determined
DESCRIPTION: Full length APOBEC3G (CEM15), c-terminus His4, produced in the baculovirus expression system.
PURIFICATION: This protein was purified by ammonium sulphate fractionation Ni-affinity chromatography to > 95% purity as determined by SDS-PAGE, reduced.. Approx MW=44kD
SPECIFICITY: This protein binds to anti-CEM15 murine monoclonal antibodies and rabbit polyclonal antibodies as determined by Elisa and Western Elisa assays.
BIOLOGICAL ACTIVITY: Binds to recombinanat HIV-1 vif as determined by ELISA and Western Blot, and complexes with native vif in HIV-1 viral lysates. Specificity for binding cellular RNA.