Item# 1019: Recombinant HIV-1C gp41 Envelope Protein (E.coli)
Concentration: See vial
Volume/vial: see vial
Diluent: 0 mM Tris, pH8,0.002%SDS
Physical State: Frozen Liquid
Stability:1 year at -20oC, 3 years at -75°C.
Application: T-Cell Activation, Immunization, In-Vitro Diagnostics.
Description: Truncated Recombinant HIV-1 C envelope protein gp41 produced in the E.coli Expression System. Truncated gp41 protein mol.wt: approx. 35kD.
Purification: This protein was purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.
Biological Activity: Not done
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Sequence bold-red below expressed in E.coli.
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