Item# 2009: Recombinant HIV-2 (Rod) gp36 Envelope Protein (E.coli)
Concentration: See vial
Diluent: 10 mM Tris, pH 8.0, SDS (<0.01%)
Physical State: Frozen Liquid
Stability: At least 24 months at -75°C.
Application: Human Diagnostics, Immunization,T-Cell Activation.
Description: Truncated Recombinant HIV-2 envelope protein gp36 produced as a fusion protein in the E.coli Expression System
Molecular Weight: 28kD
Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine anti-gp36 HIV-2 monoclonal antibodies and HIV-2 converted human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: ND
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.