rgp160 HIV-1 C (Euk)

$625.00
SKU: 1014
Size: 100 ug

Product Specifications:

Item# 1014: Recombinant HIV-1 C Glycoprotein Soluble gp160 (Baculo)                                    

 

Concentration: See Vial                                                                

 

Mass/vial:100ug/vial                                                    

 

Volume/vial: See Vial                                                         

 

Diluent: PBS                                                                                  

 

Purity: >95%                                                                      

 

Stabilizer: None                                                                       

 

Preservative: None

 

Storage: -75°C

 

Physical State: Frozen Liquid

 

Stability: 2 years at -75°C.

 

Application: CD4 Binding, Vaccines,T-Cell Activation, In-Vitro Diagnostics                         

 

Description: Full length Recombinant HIV-1 C soluble membrane anchor sequence deleted-gp160 glycoprotein produced in the Baculovirus Expression System.

 

Purification:This protein is purified by immunoaffinity Chromatography to >95% purity as determined by SDS-PAGE, reduced.

 

Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificit and human serum polyclonal antibodies in ELISA and Western ELISA.

 

Biological Activity:  This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp160/Anti gp160 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.

 

Application and Instructions for use

 

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  HIV-1 converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.