Recombinant HIV-1 C Glycoprotein Soluble gp160 (Baculo)

ImmunoDXSKU: 1014


Product Specifications:

Item# 1014: Recombinant HIV-1 C Glycoprotein Soluble gp160 (Baculo)                                    

 Concentration: See Vial                                                                


 Volume/vial: See Vial                                                         

 Diluent: PBS                                                                                  

 Purity: >95%                                                                      

 Stabilizer: None                                                                       

 Preservative: None

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: 2 years at -75°C.

 Application: CD4 Binding, Vaccines,T-Cell Activation, In-Vitro Diagnostics   

Description: Full length Recombinant HIV-1 C soluble membrane anchor sequence deleted-gp160 glycoprotein produced in the Baculovirus Expression System.

 Purification:This protein is purified by immunoaffinity Chromatography to >95% purity as determined by SDS-PAGE, reduced.

 Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificit and human serum polyclonal antibodies in ELISA and Western ELISA.

 Biological Activity:  This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp160/Anti gp160 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.

 Application and Instructions for use

 Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  HIV-1 converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.

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