rgp130 SIV mac251 (Euk)

SKU: 5001

Product Specifications:

Item# 5001: Recombinant SIVmac251 Glycoprotein gp130 (Baculovirus) 


Concentration: See vial


Mass/vial: 100µg 


Volume/vial: see vial  


Diluent: PBS


Purity: >95%


Stabilizer: None


Preservative: None


Storage: -75°C


Physical State: Frozen Liquid


Stability: At least 12 months at -75°C.


Application: Immunization, CD4 Binding, B & T-Cell Activation


Description: Full length Recombinant SIV gp130 glycoprotein produced in the Baculovirus Expression System.


Purification: This protein is purified by immunoaffinity chromatography to >90% purity as determined by SDS-PAGE, reduced.


Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and simian serum polyclonal antibodies in ELISA and Western ELISA.


Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp130/Anti-gp130 mAb-peroxidase capture ELISA. This protein activates simian T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.


Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.