rgp120 HIV-1 IIIB Conjugated to Colloidal Gold

SKU: 1001-G
Size: 1 ml

Product Specifications:

Item#1001-G: rgp120 HIV-1 IIIB Conjugated to Colloidal Gold 


Concentration: See vial 


Volume/vial: 1ml Gold Conjugate


Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3 


Storage: 4°C


Stability: At least 3 months at 4°C Minimum


Dilution: 1 : 50


Application: Dot Blot Assay-CD4 Binding, Dot Blot Assay-Anti-gp120 Antibody Binding, Immunohistochemistry. 


Description: rgp120 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).


Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.


Specificity: Gold conjugated rgp120 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays.


Biological Activity: Not determined.


Application and Instructions for use:


Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system.

2. Immunohistochemical studies may be performed in 1 : 50 dilution range.


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