ITEM # 1099: Recombinant HIVO gp41 Envelope Protein (E.coli)
Concentration: See vial
Volume/vial: see vial
Diluent: 10 mM Tris, pH 8,<0.02%SDS Purity: >98%
Physical State: Frozen Liquid
Stability: At least 24 months at -75oC.
DESCRIPTION: Truncated Recombinant HIV O envelope protein gp41 produced in the E.coli Expression System. Truncated gp41 protein mol.wt: 25kD.
APPLICATION: In-Vitro Diagnostics, Immunization, T-Cell Activation.
PURIFICATION: This protein was purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to HIVO converted human serum polyclonal antibodies in ELISA and Western Elisa.
BIOLOGICAL ACTIVITY: Not done
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.