Item# 10001: Recombinant Zika Envelope Protein (E.coli)
Concentration: See vial
Mass/vial : 1mg
Diluent :50mM NaCl, 50mM NaPO4, pH8.5 0.02%SDS
Purity : >95%
Physical State: Frozen Liquid
Stability: At least 2 years at -750C
Application: Elisa/Western Elisa, Diagnostics.
Description: Synthetic Zika Envelope DNA sequence expressed in the E.coli Expression System.
Purification: This protein was purified by aqueous solvent extraction and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 49kD.
Specificity: This protein binds to Zika converted human serum polyclonal antibodies and cross reacts with Dengue virus converted human serum polyclonal antibodies as determined by Elisa and Western analyses. Zika specific monoclonal antibody bimding analysis pending.
Application and Instructions for use
Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Concentarions if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.