ITEM# 5009: Recombinant SIV gp41 Envelope Protein (E.coli)
Concentration: See vial
Volume/vial: see vial
Diluent: 50 mM Tris, 0.1M NaCl, 2mM DDT, pH8
Physical State: Frozen Liquid
Stability: At least 24 months at -75 oC.
DESCRIPTION: Truncated Recombinant SIV gp41 envelope protein produced in the E.coli Expression System. Truncated gp41 protein mol.wt: 28kD.
Applications : In- Vitro Diagnostics,Immunization, Elisa/Western Blots.
PURIFICATION: This protein was purified by solvent extraction and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to HIV-2 converted human serum polyclonal antibodies in ELISA and Western Elisa.
BIOLOGICAL ACTIVITY: Not done
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.
Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard