Item# 1093: Recombinant HIV-1 IIIB gag p55
Concentration: See vial
ELISA Mass/vial: 0.1mg
Diluent: Na-Ac, 20mM,0.15M NaCl, pH.6.5
Purity : >95%
Physical State: Frozen Liquid
Stability: At least 24 months at -75oC.
Application: Elisa, Western ELISA, In-Vitro HIV-1 Diagnostics .
Description: Recombinant HIV-1 IIIB gag p55 is a cytosolic protein produced in the Baculovirus Expression System.
Purification: This protein was purified by cation/anion affinity and gel exclusion chromatography to >95% purity as determined by SDS-PAGE, reduced. MW: approx 55kD.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and to rabbit and human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Not Determined
Applications and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA may be performed at 10-100ng protein depending on the nature and affinity of the detection reagent. HIV –1 converted uman serum polyclonal antibodies yield titers of up to 1:100,000 at 10 -100ng of immobilized protein under standard ELISA conditions.