Item# 30190: Recombinant Dengue Antigen D4 Envelope Protein (Baculo)
Concentration: See vial
Mass/vial: See vial
Diluent: 5mM Hepes, pH8.8, 7.5mM NaCI
0.5% Glycerol, 0.02M Beta ME
0.01% Triton X IOO
Storage: -750C, 20°C
Physical State: Liquid
Stability: At least 36 months at -75°C.
Applications: Elisa/Western Elisa, Diagnostics
Description: Synthetic Dengue virus D4 Envelope DNA sequence, expressed in the Baculo Expression System.
Purification: This protein is purified by Ni affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 43.5kD.
Specificity: This protein binds to Dengue virus converted human sera as determined by Elisa, Western Blots and by one step lateral flow diagnostic testing.
Application and Instructions for use
Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.