rDengue Antigen D4 Envelope (E. coli)

SKU: 30090

Product Specifications:

Item# 30090: Recombinant Dengue D4 Envelope Protein (E.coli)


Concentration: see vial


Mass/vial: see vial 


Solids: 50mM NaCl,50mM Tris, pH8.5, 0.02%SDS


Purity: >95%


Stabilizer:  None


Preservative: None


Storage: -20 -750C


Physical State: Frozen  Liquid


Stability: At least 36 months at -750C


ApplicationsElisa/Western Blot, Dengue Diagnostics


Description: Synthetic Dengue virus D2 Envelope DNA sequence, amino acid residues 1-416, expressed as a fusion protein in the E.coli Expression System.


Purification: This protein is purified by Ni affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.


Molecular Weight: approx. 75kD.


Specificity: This protein binds to murine monoclonal antibodies – structural epitopes, and to Dengue virus converted human serum antibodies as determined by Elisa, Western Blots and by one-step lateral flow diagnostic testing.


Application and Instructions for use:

Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Concentarions if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.