Item# 30070: Recombinant Dengue D2 Envelope Protein (E.coli)
Concentration: See vial
Mass/vial : See vial
Diluent : 50mMTris, pH8, 10mM NaCl
Purity : >95%
Stabilizer : None
Physical State:Frozen Liquid
Stability: At least 36 months at -750C.
Applications: Elisa/Western Elisa, Diagnostics
Description: Synthetic Dengue virus D2 Envelope DNA sequence, amino acid residues 1-428, expressed in the E.coli Expression System.
Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 49kD.
Specificity: This protein shows strong reactivity with Dengue virus converted human serum antibodies as determined by Elisa and Western Blot assays. Useful for Dengue serum IgG/IgM detection.
Application and Instructions for use
Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.