rDengue Antigen D2 Envelope (E. coli)

SKU: 30070-10

Product Specifications:

Item# 30070: Recombinant Dengue D2 Envelope Protein (E.coli)    


Concentration: See vial


Mass/vial : See vial 


Diluent : 50mMTris, pH8, 10mM NaCl


Purity : >95%


Stabilizer : None


Preservative: None


Storage: -750C


Physical State:Frozen Liquid


Stability: At least 36 months at -750C.


Applications:  Elisa/Western Elisa, Diagnostics


Description: Synthetic Dengue virus D2 Envelope DNA sequence, amino acid residues 1-428, expressed in the E.coli Expression System.


Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to  >95% purity as determined by SDS-PAGE, reduced. 


Molecular Weight: approx. 49kD.


Specificity: This protein shows strong reactivity with Dengue virus converted human serum antibodies as determined by Elisa and Western Blot assays. Useful for Dengue serum IgG/IgM detection.


Appliucation and Instructions for use

Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Concentarions if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.