PRODUCT # 135-10: Rabbit Anti-rTB ESAT-6 Antigen Polyclonal Antibodies
Concentration: 1mg/ml ESAT-6 ELISA
Mass/vial: 0.1mg/100ul ESAT-6 Western ELISA
Diluent: PBS Immunoprecipitation
Purity: >95% Human TB Diagnostics
Physical State: Frozen Liquid
Stability: At least 24 months
Minimum Dilution: 1 : 1000
DESCRIPTION: pAb IgG fraction purified from rTB ESAT-6 antigen converted rabbit immune serum.
PURIFICATION: This pAb IgG was purified by protein A/G affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This pAb IgG binds to native and recombinant TB antigen ESAT-6 as determined by ELISA and Western ELISA. Useful in capturing ESAT-6 specific polyclonal antibodies from human active TB serum in a twin site assay using anti-ESAT-6 murine monoclonal antibody (product # 115-10) and rabbit anti-ESAT-6 polyclonal Antibody-HRP as a detection reagent ( product # 135-10P).
APPLICATION AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized rESAT-6(Product# 135-10) under standard ELISA conditions. ELISA and Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen.
Product# 135-10-P: Peroxidase-Conjugated Rabbit anti-ESAT-6 pAb
Application: Diagnostics, ELISA, Western ELISA.
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