PRODUCT # 135-20: Rabbit Anti-rTB CFP-10 Antigen Polyclonal Antibodies
Concentration: 1mg/ml CFP-10 ELISA
Mass/vial: 0.1mg/100ul CFP-10 Western ELISA
Diluent: PBS Immunoprecipitation
Purity: >95% Human TB Diagnostics
Physical State: Frozen Liquid
Stability: At least 24 months
Minimum Dilution: 1 : 1000
DESCRIPTION: pAb IgG fraction purified from rTB CFP-10 antigen converted rabbit immune serum.
PURIFICATION: This pAb IgG was purified by protein A/G affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This pAb IgG binds to native and recombinant TB antigen CFP-10 as determined by ELISA and Western ELISA. Useful in capturing CFP-10 specific polyclonal antibodies from human active TB serum in a twin site assay using anti-CFP-10 murine monoclonal antibody (product # 115-20) and rabbit anti-CFP-10 polyclonal antibody-HRP as a detection reagent ( product # 135-20-P).
APPLICATION AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized rESAT-6(Product# 135-20) under standard ELISA conditions. ELISA and Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen.
Product#135-20-P: Peroxidase-Conjugated Rabbit anti-ESAT-6 pAb
Product #145-20: Goat anti-CFP-10 Polyclonal Antibody
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