Rabbit pAb CFP-10

$425.00
SKU: 135-10
Size: 100 ug

PRODUCT SPECIFICATIONS

PRODUCT# 1052:  Recombinant tat HIV-1 Bal

                                                       

SPECIFICATIONS                                                               APPLICATIONS

  Concentration:                 1mg/ml                                                                ELISA

  Mass/vial:                          100ug                                                              Western ELISA

  Volume/vial:                     See vial                                                           Anti-tat Drug Screening

  Diluent:                               50mM Tris, 0.1M NaCl, 2mMdtt, pH7.8          Immunization

  Purity  :                               >95%                                                                       Transcriptional Activation              

  Stabilizer:                          None                                                                        

  Preservative:                     None                                                                     

  Storage:                              -75oC

  Physical State:                  Frozen Liquid

  Stability:                            At least 6 months at -75oC.             

                                              

DESCRIPTION:                   Full length (101 amino acids) Recombinant HIV-1 Bal tat produced in the E.                                                                                       coli Expression System.

 

PURIFICATION:                 This protein was  purified by solvent extraction, ion affinity and macro-molecular                                                                            sieving  to approx.95% purity, as determined by SDS-PAGE , reduced. MW: approx.                                                                          16kD.

 

SPECIFICITY:                       This protein binds to murine monoclonal antibodies of defined epitope specificity                                                                     and rabbit and human serum polyclonal antibodies in ELISA and Western ELISA.

 

BIOLOGICAL ACTIVITY:  Not Done.

 

ENDOTOXIN:                         Assay Pending.

 

APPLICATION AND INSTRUCTIONS FOR USE

 

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.  Dilute tat stock solution in Tris- saline or salins-phosphate buffer, pH 7-8,  immediately before use.  Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity.

 

                Transcriptional activation assays with tat are performed in 1-10µg/ml range.  ELISA and Western ELISA require tat in 10-100ng protein range.