Item#1310: Rabbit Anti-Protease HIV-1 HXB2 pAb IgG
Concentration: See vial
Physical State: Frozen Liquid
Stability: At least 12 months at -75°C
Minimum Dilution: 1 : 1000
Application: Elisa/Western Elisa, Neutralization, Immunoprecipitation, Immunohistochemistry.
Description: pAb IgG fraction purified from Protease HIV-1 immunized rabbit serum .
Purification: This pAb IgG was purified by protein-A affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This pAb IgG binds to native and recombinant HIV-1protea se in ELISA and Western ELISA.
Biological Activity: Not Determined
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized protease (Product# 1010) under standard ELISA conditions. ELISA and Western ELISA may be performed at 1- 2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen.