Item# 1121-P: Peroxidase-Conjugated Murine Anti-gp120 mAb
Stabilizer: 50mM Tris, pH 8,1%BSA
Preservative: 0.01% Thimerasol
Physical State: Liquid
HRP: 2 - 3
Stability: At least 3 months at 4°C
Minimum Dilution: 1 : 2000
Application: Dot Blots, Immunohistocytology, gp120 Capture Assays, Drug Screening, Human T-cell Research
Description: Murine Anti-gp120 mAb IgG (high affinity, cross reactive) conjugated to peroxidase.
Molar Ratio: 2 - 4 peroxidase molecules per molecule of mAb.
Specificity: Peroxidase conjugated Murine anti-gp120 mAb binds to native and recombinant gp120 (V3 loop specific) in ELISA and Western ELISA.
Biological Activity: Peroxidase conjugated Murine anti-gp120 mAb binds to gp120 (IIIB, MN, SF2, RF, CDC4) in CD4/gp120 capture ELISA and Western ELISA.
Application and Instructions for use
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA and Western ELISA may be performed at dilutions of 1 : 5000 depending on the concentration of gp120 in the test system. CD4/gp120 capture ELISA may be performed in 1 : 2000 dilution range depending on the capture ligand CD4 concentration and gp120 concentration in the test sample. Typical assays with 100ng of solid phase CD4 will detect at least 100ng/ml gp120.