Product # 4005: Recombinant HCV 19kD NS4 Protein
Concentration: see vial In-Vitro HCV Diagnostics
Mass/vial: see vial Western Analysis
Volume/vial: see vial
Diluent: 20mM Tris, pH8.0,10mM Beta ME 4M Urea
Purity : >95%
Storage: -75o C
Stability: At least 24 months at -75o C
DESCRIPTION: NS4 fragment - 130 aminoacids of HCV polyprotein expressed in the E. coli expression system. MW: approx. 20kD
PURIFICATION: HCV NS4 protein is purified by affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
SPECIFICITY: This protein binds to murine monoclonal antibodies specific for HCV NS4 protein. NS4 protein shows strong binding to HCV converted human serum polyclonal antibodies.
BIOLOGICAL ACTIVITY: Not determined.
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10 to 20-fold in PBS for solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
HCV core protein shows relatively strong biding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.