Product# 1101: Murine anti-gp120 IIIB Monoclonal Antibody
Antibody Class: IgG1 ELISA, Western ELISA
Concentration: See vial Immunoprecipitation
Mass/vial: 100µg Immunocytochemistry
Purity: >95% Immunohistochemistry
Stabilizer: None In Vitro Virus Neutralization
Physical State: Frozen Liquid
Stability: At least 12 months at -75oC
DESCRIPTION: High affinity murine anti-gp120 IIIB mAb.
SPECIFICITY: Binds to V3-loop of HIV-1 IIIB gp120/gp160. This mAb shows 5-10% cross reactivity with other HIV-1 strains.
ACTIVITY: Neutralizes HIV-1 IIIB at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 IIIB infected cells and co-cultures of infected PBLs.
APPLICATIONS AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml.
PRODUCT#: 1101-P Peroxidase-Conjugated Murine anti-gp120 HIV-1 IIIB
Application: ELISA, Western ELISA, Histocytochemistry, Histocytology.
PRODUCT#: 1101-F Fluorescene Murine anti-gp120 HIV-1 IIIB
Application: FACS, Cell Staining
PRODUCT#: 1101-G Colloidal Gold-Conjugated Murine anti-gp120 HIV-1 IIIBApplication: Dot Blots, EM.