Leptospira Composite L2 Antigen

SKU: 90020
Size: 1 mg

Product Specifications:

Item# 90020: Recombinant LeptospiraComposite L2 Antigen OmpL1-MLP17-LigA (E.coli)


Concentration:  See Vial                                                  


Mass/vial: See vial                                                


Volume/vial: See Vial                                                          


Diluent: 50mM Tris, 50mM NaCl, pH9, 10%Glycerol                                                                                


Purity: Approx. 98%                                                                                                    


Stabilizer: None                                                                        




Storage: -75°C


Physical State: Frozen Liquid


Stability: 2 years at -75°C 


Application: In-Vitro Diagnostics, Immunization.


Description:Full length Recombinant Leptospira Composite L2 antigen- LipL32-LipL41-produced in the E.coli Expression System.


Purification:This protein is purified by solvent extraction, preparative electrophoresis and fusion tag affinity immuno-chromatography (proprietary) to >98% purity, as determined by SDS-PAGE, reduced

 Approx. Molecular Weight: 62.2kD.


Specificity: This protein binds to Leptospira converted human serum polyclonal antibodies, as determined by ELISA and Western   ELISA. Suitable for use in lateral flow human Leptospira diagnostics.


Biological Activity: Not done


Application and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Leptospira converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.