Item # 2506: Human Anti-Reverse Transcriptase HIV-1 (IIIB) pAb IgG
Concentration: See vial
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 1000
Application: ELISA/RT Western ELISA, Immunoprecipitation, Immunohistochemistry, Drug Screening.
Description: pAb IgG fraction purified from HIV-1 immune human serum .
Purification: This pAb IgG was purified by RT affinity chromatography to >90% purity as determined by SDS-PAGE, reduced.
Specificity: This pAb IgG binds to native and recombinant HIV-1 RT in ELISA and Western ELISA. Cross reactivity with HIV-1 envelope and core proteins not detected.
Biological Activity: None
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized gp41 (Product# 1091) under standard conditions. ELISA and Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen Histochemical studies require dilutions in 1 : 200 dilution range.
Caution: This product is derived from HIV-1 infected human serum. This product must be used by qualified personnel only under strict guidelines for the use of infectious agents. Use of syringes with this product is forbidden.