Item # 2501: Human Anti-gp120/160 HIV-1 pAb IgG
Concentration: See vial
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 1000
Application: ELISA/gp120 Western ELISA, Immunoprecipitation, Immunohistochemistry, Virus Neutralization Assays, FACS, gp120 Capture ELISA.
Description: Affinity purified pAb IgG fraction from HIV-1 immune human serum .
Purification: This pAb IgG was purified by rgp120-affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This pAb IgG binds to native and recombinant HIV-1 gp120/160 in ELISA and Western ELISA.
Biological Activity:Neutralizes HIV-1 virus in co-cultures as assayed by syncycium formation and in supT1 infected cells by p24, RT, LTR-CAT activation and cell viability assays.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized gp120/160 (IDI Product# 1001, 1004) under standard conditions. ELISA and Western ELISA may be performed at 1-5µg/ml of pAb depending on the nature and the quantity of the immobilized antigen. Histochemical studies require dilutions in 1 : 200 dilution range. Virus neutralization assays may be performed in 0.1 to 10µg/ml range.
Caution: Use of needles for handling and transfer of this product is not recommended, and ensure proper laboratory safety precautions are in place for handing and use of this product.