Item # 2500: Human Anti HIV-1 pAb IgG
Concentration: See vial
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 1000
Application: ELISA/gp120 Western ELISA, Immunoprecipitation, Immunohistochemistry, Virus Neutralization Assays, FACS, gp120 Capture ELISA.
Description: Protein A/G affinity purified pAb IgG fraction from HIV-1 immune human serum .
Purification: This pAb IgG was purified by proteinA/G affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This pAb IgG binds to native and recombinant HIV-1viral protein lysates as determined by ELISA and Component HIV-1 envelope, core and regulatory proteins as determined by Western ELISA.
Biological Activity: Neutralizes HIV-1 virus in co-cultures as assayed by syncycium formation and in supT1 infected cells by p24, RT, LTR-CAT activation and cell viability assays.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized gp120/160 (IDI Product# 1001, 1004) under standard conditions. ELISA and Western ELISA may be performed at 1-5µg/ml of pAb depending on the nature and the quantity of the immobilized antigen. Histochemical studies require dilutions in 1 : 200 dilution range. Virus neutralization assays may be performed in 0.1 to 10µg/ml range.
Caution: This product is dervived from HIV-1 infected, converted human serum. Use of needles for handling and transfer of this product is not recommended. This product must be used by trained professional only, following all the necessary bio-safety precautions and procedures.