Item# 3501: Human Anti-gp120 HIV-1 Monoclonal Antibody
Antibody Class: IgG
Concentration: See vial
Physical State: Frozen Liquid
Stability: At least 12 months at -75°C
Application: ELISA, Western ELISA, Immunoprecipitation, Immunohistochemistry, HIV-1 Neutralization,
Description: High affinity Human anti-gp120 HIV-1 mAb. Human-mouse chimera produced in human myeloma cell line in culture.
Specificity: Binds to CD4 binding region of gp120.
Biological Activity: Neutralizes HIV-1 IIIB at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 IIIB infected cells and co-cultures of infected PBLs.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. Neutralization assays are performed in 1-10µg/ml range depending on virus TCID.
Caution: Avoid use of needles and ensure proper laboratory safety precautions are in place for the use of this product.
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